On October 22, 2008, the Department of Transportation issued a Federal Register Notice concerning direct observation (DO) of urine specimens collected for return-to-duty and follow-up drug testing. The effective date of 49 CFR 40.67(b), as amended by the Department on June 25, 2008, and delayed on August 26, 2008, is November 1, 2008. Beginning that date [November 1], DO collections will be required for all return-to-duty and follow-up DOT tests.